FIELD: biotechnology.
SUBSTANCE: described is a method of purifying a polyspecific IgG antibody from a mixture using affinity chromatography. Method comprises immobilizing a polyspecific IgG antibody from a mixture on a first affinity chromatography column and elution of the polyspecific IgG antibody with an elution buffer, containing an antiaggregation composition for purifying a polyspecific IgG antibody from a mixture, where the polyspecific IgG antibody is an antibody consisting only of heavy chains or a three-chain antibody-like molecule.
EFFECT: invention extends the range of means for purifying three-chain antibodies or three-chain antibody-like molecules and reduces aggregation during purification thereof.
20 cl, 17 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
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| BINDING MOLECULES INDUCING CELLULAR ACTIVATION | 2019 |
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| READILY ISOLATED BISPECIFIC ANTIBODIES WITH NATIVE IMMUNOGLOBULIN FORMAT | 2010 |
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