FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. Disclosed is a method of converting lignocellulose in an organic acid. Method comprises pretreating lignocellulose material with an alkaline agent comprising containing calcium or magnesium cations in presence of water at a temperature of pretreatment from 20 to 115 °C. Aqueous suspension of pretreated material is obtained. At least part of suspension is fed into a fermentation zone. Method includes enzymatic hydrolysis and fermentation to produce broth containing insoluble lignocellulose, precipitated and dissolved calcium or magnesium salt of an organic acid and an enzyme. Enzymatic broth is unloaded, followed by separation of a liquid phase containing dissolved salt of an organic acid, and a solid phase containing insoluble lignocellulose and a precipitated salt of an organic acid. At least part of liquid phase is recycled to pretreatment step with alkali, and/or to fermentation zone.
EFFECT: invention enables to obtain a product in highly concentrated form with high output and purity.
16 cl, 1 dwg
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