APPARATUS FOR SYNTHESIS OF OLIGO(POLY)NUCLEOTIDES Russian patent published in 2016 - IPC C12M1/00 C12M1/04 

FIELD: biology.

SUBSTANCE: invention relates to devices for synthesis of DNA and RNA molecule fragments in automatic mode, is used in molecular biology, genetic engineering, medicine. Apparatus for synthesis of oligo(poly)nucleotides comprises a reaction column with a solid-phase carrier placed therein, container with reagents for synthesis and auxiliary reagents, means of feeding reactants into reaction column and means to remove reagents therefrom, excess pressure gas source, vessel with flushing liquid, gas and fluid valves. Device is equipped with control and monitoring means in form of a programmable computer. Reaction column is in form of cylindrical container with a piston located in cavity thereof, installed with possibility of movement along axis of vessel. Reaction column is equipped with piston position sensor and drive and has at least two inputs for supply and removal of reactants. One of inputs is located in piston, and second is located opposite - in base of cylindrical vessel.

EFFECT: higher quality of synthesis of oligo(poly)nucleotides using solid-phase carrier with possibility of changing their volume during synthesis and reduced consumption of reactants.

4 cl, 1 dwg