ORGANOID-DIRECTED NANOCARRIERS Russian patent published in 2016 - IPC C12N15/87 A01H5/00 A01K67/27 C07K14/415 C07K7/08 C12N15/00 

FIELD: biology.

SUBSTANCE: invention relates to genetic engineering. Described is a method of delivery of nucleic acid to a mitochondrion and of genetic modification of a cell, including impact on the cell by a composition containing at least one nucleic acid and at least one organoidly-directed applicator, where the applicator delivers at least one nucleic acid through the cell membrane to a mitochondrion. Organoidly-directed applicator is a polypeptide having directed to a mitochondrion peptide sequence, a charge from approximately 4 to approximately 7 and hydrophilic nature from approximately 0 to approximately -0.5. There is presented an applicator directed to a mitochondrion and being a polypeptide containing a peptide sequence, directed to a mitochondrion, a charge in the range from approximately 4 to approximately 7 and hydrophilic nature from approximately 0 to approximately -0.5. There is described a method for identification of a directed on a mitochondrion polypeptide applicator, including selection from the database of peptide sequences of the first subgroup of signal peptide sequences, specific towards mitochondria, selection from the first subgroup of signal peptide sequences of the second subgroup of peptide sequences having a positive net charge of at least 3.5, selection from the second subgroup of peptide sequences of the third subgroup of peptide sequences having an average hydrophilic nature of maximum 0. Then, a cell and a peptide are brought into contact; at that, the peptide has the sequence selected from the third subgroup of peptide sequences, and there is performed identification of the peptide as of a polypeptide applicator targeted on a mitochondrion, if the peptide penetrates through the cell membrane and localises with the mitochondrion in the cell.

EFFECT: described is a method of obtaining a genetically modified plant, including preparation of a genetically modified plant cell using the described method and growing of the plant from the genetically modified plant cell.

16 cl, 20 dwg, 9 tbl, 5 ex