FIELD: chemistry; biochemistry.
SUBSTANCE: present invention relates to a novel fluorescent protein from Entacmaea quadricolor and its functional mutants. The invention discloses nucleic acids which code the said protein, a vector which contains the said nucleic acids, a transgenic cell which carries the vector and a method of obtaining the said fluorescent proteins from transgenic cells. The composition of the said proteins and nucleic acids can be used in various applications and methods, particularly for labelling biomolecules, cells or cell organelles. The disclosed protein and nucleotide sequences can be used for testing activity of promoters under various conditions.
EFFECT: obtaining proteins with primarily red or far-red fluorescence.
7 cl, 7 dwg, 7 ex
| Title |
Year |
Author |
Number |
| FLUORESCING PROTEINS AND CHROMOPROTEINS FROM KINDS HYDROZOA WHICH ARE NOT CONCERNING TO AEQUOREA, AND METHODS OF THEIR OBTAINING |
2003 |
- Luk'Janov Sergej Anatol'Evich
- Shagin Dmitrij Alekseevich
- Janushevich Jurij Grigor'Evich
|
RU2338785C2 |
| ISOLATED NUCLEIC ACID, ENCODING OPERATIVELY FUSED INTRAMOLECULAR DIMER OF KillerRed PROTEIN, EXPRESSION CASSETTE, CELL, WHICH PRODUCES CHIMERIC PROTEIN AND CONTAINS EXPRESSION CASSETTE, ISOLATED CHIMERIC PROTEIN |
2011 |
- Luk'Janov Konstantin Anatol'Evich
- Serebrovskaja Ekaterina Olegovna
- Luk'Janov Sergej Anatol'Evich
|
RU2491342C1 |
| ISOLATED KillerRed PROTEIN-CODING NUCLEIC ACID (VERSIONS), ISOLATED PROTEIN (VERSIONS), EXPRESSION CASSETTE, CELL CONTAINING EXPRESSION CASSETTE |
2011 |
- Luk'Janov Konstantin Anatol'Evich
- Serebrovskaja Ekaterina Olegovna
- Luk'Janov Sergej Anatol'Evich
|
RU2458129C1 |
| MODIFIED GENETICALLY CODED PHOTOSENSITIZER |
2013 |
- Sarkisyan Karen Sergeevich
- Mishin Aleksandr Sergeevich
- Lukyanov Sergej Anatolevich
|
RU2599443C2 |
| NUCLEIC ACID ENCODING FRET-BASED FAR-RED BIOSENSOR FOR INTRACELLULAR CASPASE 3 ACTIVITY MEASUREMENT |
2013 |
- Luk'Janov Konstantin Anatol'Evich
- Zlobovskaja Ol'Ga Anatol'Evna
|
RU2535981C1 |
| CHIMERIC PROTEIN BEING FLUORESCENT BIOSENSOR FOR SIMULTANEOUS DETECTION OF HYDROGEN PEROXIDE AND PHOSPHATIDYL INOSITOL-3,4,5-TRIPHOSPHATE, NUCLEINIC ACID CODING SUCH PROTEIN, EXPRESSION CASSETTE AND EUCARIOTIC HOST CELL |
2012 |
- Belousov Vsevolod Vadimovich
- Mishina Natalija Mikhajlovna
- Chudakov Dmitrij Mikhajlovich
|
RU2498996C1 |
| MODIFIED BIOSENSOR FOR DETECTING INTRACELLULAR PH |
2014 |
- Belousov Vsevolod Vadimovich
- Matlashov Mikhail Egorovich
- Zagajnova Elena Vadimovna
- Ermakova Julija Gennadevna
- Lukjanov Sergej Anatolevich
|
RU2603060C2 |
| ISOLATED NUCLEIC ACID CODING FLUORESCENT BIOSENSOR FOR DETECTION OF HYDROGEN PEROXIDE, EXPRESSION CASSETTE, CELL THAT PRODUCES BIOSENSOR, ISOLATED FLUORESCENT BIOSENSOR FOR DETECTION OF HYDROGEN PEROXIDE, ISOLATED NUCLEIC ACID THAT CODES FLUORESCENT BIOSENSOR, EFFICIENTLY FUSED WITH NUCLEIC ACID THAT CODES SIGNAL OF INTRACELLULAR LOCALISATION |
2011 |
- Bilan Dmitrij Sergeevich
- Belousov Vsevolod Vadimovich
- Luk'Janov Sergej Anatol'Evich
|
RU2493260C2 |
| ISOLATED NUCLEIC ACID CODING FLUORESCENT BIOSENSOR, EXPRESSION CASSETTE, CELL, PRODUCING FLUORESCENT BIOSENSOR, ISOLATED FLUORESCENT BIOSENSOR |
2012 |
- Bilan Dmitrij Sergeevich
- Belousov Vsevolod Vadimovich
- Luk'Janov Sergej Anatol'Evich
|
RU2515903C2 |
| MODIFIED GREEN FLUORESCENT PROTEINS AND METHODS OF THEIR APPLICATION |
2006 |
- Luk'Janov Sergej Anatol'Evich
|
RU2412250C2 |
