FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and can be used for recombinant production of mutants of phototoxic fluorescent protein. Mutants of KillerRed phototoxic fluorescent protein are obtained by recombinant production. KillerOrange mutant with SEQ ID NO: 02 comprises replacements Y68W, D115G, N147S, F179L, Y223H and E238Q, wherein KillerGreen with SEQ ID NO: 04 comprises replacements V46A, Y68W, A74T, D115G, N147S, Y223H and E238Q.
EFFECT: invention enables to obtain mutants of KillerRed phototoxic fluorescent protein having changed spectral characteristics.
6 cl, 3 dwg, 1 tbl, 5 ex
| Title |
Year |
Author |
Number |
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2011 |
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2012 |
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RU2498996C1 |
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2014 |
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2013 |
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RU2434943C2 |
