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2 595 427

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(51)

IPC

C12Q1/68(2006-01-01)

(21) (22)

Application

2015138770/10, 2015-09-11

(24)

Start date

2015-09-11

(22)

Actual filing date

2015-09-11

(45)

Published

2016-08-27

(72)

Inventor

Kosovskij Gleb YurevichKovalchuk Svetlana NikolaevnaGlazko Tatyana Teodorovna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Nauchnoe Uchrezhdenie Eksperimentalnoj Embriologii I Reproduktivnykh Biotekhnologij" Tseerb)

SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES FOR DETECTION OF msp4 GENE OF Anaplasma marginale RICKETTSIAE BY POLYMERASE CHAIN REACTION IN "REAL TIME" Russian patent published in 2016 - IPC C12Q1/68

Abstract RU 2595427 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, molecular genetic diagnostics, veterinary medicine, in particular to a set of oligodesoxyribonycleotides for amplification and detection of species-specific DNA Anaplasma marginale fragment by polymerase chain reaction (PCR) in real time. Kit consists of two oligodeoxyribonucleotide primers: F: 5-CATGAGTCACGAAGTGGCT-3′, R: 5′-GGCACACTCACATCAATC-3′ and one fluorescent-marked DNA probe: 5′-Cy5-AAGGGGGAGTAATGGGAGGTAGCT-BHQ2.

EFFECT: higher reliability of detecting Anaplasma marginale, simplification of analysis and its shorter time, by up to 1½ hours (excluding sample preparation).

1 cl, 3 dwg, 1 tbl

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RU 2 595 427 C2

Authors

Kosovskij Gleb Yurevich

Kovalchuk Svetlana Nikolaevna

Glazko Tatyana Teodorovna

Dates

2016-08-27—Published

2015-09-11—Filed