FIELD: veterinary medicine.
SUBSTANCE: MSP4 gene fragment encoding anaplasmas DNA is identified, using 10 different pairs of forward and reverse oligonucleotide primers. Electrophoretic determination of the size of the nucleotide sequence amplified fragment is carried out. According to the presence of fragments of appropriate length on the electrophoregram, Anaplasma marginale and Anaplasma ovis are diagnosed in the animal blood.
EFFECT: effective method for Anaplasma marginale and Anaplasma ovis genomic fragments detection by PCR using specific oligonucleotide primers.
3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES FOR DETECTION OF msp4 GENE OF Anaplasma marginale RICKETTSIAE BY POLYMERASE CHAIN REACTION IN "REAL TIME" | 2015 |
|
RU2595427C2 |
| SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES FOR DETECTION OF msp1α RICKETTSIA Anaplasma marginale GENE BY "REAL TIME" POLYMERASE CHAIN REACTION | 2015 |
|
RU2603254C2 |
| STRAIN ANAPLASMA OVIS LESTOQUARD, 1924, KADOM_1, USED FOR PRODUCTION OF MEANS DIAGNOSTIC AND SPECIFIC PREVENTION OF ANAPLASMOSIS IN SHEEP AND GOATS | 2023 |
|
RU2817567C1 |
| ANAPLASMA SPECIOSUS OMSK STRAIN OF NEW GENOTYPE USED FOR IDENTIFYING ANAPLASMA AND MAKING DIAGNOSTIC PREPARATIONS | 2008 |
|
RU2393211C2 |
| METHOD FOR LEUKOSIS DIAGNOSIS IN CATTLE | 2016 |
|
RU2644233C2 |
| METHOD FOR DETECTION OF BOVINE LEUKOSIS VIRUS (BLV) PROVIRUS DNA IN FOOD BY REAL-TIME POLYMERASE CHAIN REACTION | 2022 |
|
RU2794654C1 |
| METHOD FOR DETECTING PROVIRUS DNA OF BOVINE LEUKOSIS VIRUS (BLV) | 2018 |
|
RU2700245C1 |
| TEST SYSTEM FOR THE DETECTION OF BOVINE LEUKOSIS VIRUS (BLV) DNA IN FOOD BY REAL-TIME POLYMERASE CHAIN REACTION | 2022 |
|
RU2782573C1 |
| METHOD FOR DETECTING DNA OF RHINOTRACHEITIS VIRUS (BOVINE HERPES VIRUS 1, BOHV-1) IN CATTLE | 2018 |
|
RU2700449C1 |
| DIAGNOSTIC TECHNIQUE FOR CATTLE LEUKAEMIA BY POLYMERASE CHAIN REACTION | 2010 |
|
RU2445370C1 |
