METHOD FOR STIGMINES GROUPS QUANTITATIVE DETERMINATION Russian patent published in 2016 - IPC G01N33/15 G01N33/52 

FIELD: chemistry.

SUBSTANCE: invention relates to analytical chemistry and method for quantitative determination of stigmines group in pharmaceutical ingredients. Method summary is in, that analyzed sample is added with 20-30 ml of purified water for aminostigmine, rivastigmine, pyridostigmine bromide or ethyl alcohol 95 % for methyl sulphate neostigmine and physostigmine salicylate. Then mixture is maintained at room temperature and same solvent is added to mark with further addition to aliquot part of the prepared solution of KOH alcohol solution, heated for 10 minutes, cooled and treated with alkaline solution of diazo reactant with 2.5 ml of 0.1 M NH₄OH solution and colored solutions photoelectric colorimetry, comparison solution - diazotized n-anisidine solution and potassium hydroxide.

EFFECT: use of the method enables high-accuracy determination of quantitative content of stigmines in pharmaceutical ingredients.

1 cl, 7 tbl, 1 ex