METHOD FOR QUANTITATIVE DETERMINATION OF DERIVATIVES OF IMIDAZOLE UNSUBSTITUTED IN 5-POSITION Russian patent published in 2017 - IPC G01N33/00 

FIELD: pharmacology.

SUBSTANCE: for test solutions preparation, the exact volume of 4% histidine hydrochloride amidine solution (1 ml) is placed in a 25 ml flask in 10 ml of purified water, mixed and adjusted with the same solvent; an accurately measured volume of 0.1% histamine dihydrochloride (1 ml) or precise weights of clotrimazole (about 0.1 g), thiamazole (about 0.005 g), ozagrel (about 0.01 g), bifonazole (about 0.005 g) are placed in 50 ml volumetric flasks, dissolved in methanol at a room temperature until complete dissolution, and then the flasks volume is adjusted with the same solvent. Then, 1.0, 2.0, 3.0, 4.0, 5.0 ml of the prepared solution of histidine hydrochloride and clotrimazole, 5.0, 5.5, 6.0, 6.5, 7.0 ml of dihydrochloride histamine solution, 2.0, 2.5, 3.0, 3.5, 4.0 ml of thiamazole solution, 1.0, 1.5, 2.0, 2 5, 3.0 ml of ozagrel solution and 4.0, 5.0, 6.0, 7.0, 8.0 ml of bifonazole solution are accurately selected into 20 ml volumetric flasks. 5.5 ml of a diazotized p-anisidine solution in hydrochloric acid is added to each flask, and adjusted with methanol, staining appears. Bright red colored solutions obtained after 2-3 minutes are stable for 2 hours. The samples are subjected to photoelectrocolorimetry at a wavelength of 490 nm in a 10 mm thick cuvette. The number of defined drugs is calculated using calibration curves. A solution of p-anisidine diazotized in hydrochloric acid is used as a reference solution.

EFFECT: invention provides a simple, rapid and reproducible method for quantitative determination of imidazole derivative drugs.

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