FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and represents method of producing nitrile hydratase, involving following steps: stage of cultivation of microorganisms, having activity nitrile hydratase, protected from light conditions, stage of destruction of obtained by culturing microbial cells using high-pressure homogenizer, step for storing obtained as described above, destroyed microbial cells at 4-37 °C in conditions of their protection from effect of light, and step of obtaining supernatant containing nitrile hydratase. Invention also relates to method of storing a nitrile hydratase, method of activating nitrile hydratase and to method of producing activated nitrile hydratase.
EFFECT: invention provides simple method of storing enzyme from microbial cell culture, produced during cultivation, as well as increasing enzyme activity during such preservation.
8 cl, 6 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PRODUCING AMIDE COMPOUND USING BACTERIAL CATALYST | 2001 |
|
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| RHODOCOCCUS RHODOCHROUS NCIMB 41164 STRAIN AND USE THEREOF AS NITRILE HYDRASE PRODUCER | 2004 |
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| STRAIN OF BACTERIA PHODOCOCCUS RHODOCHROUS - A PRODUCER OF MITRILE HYDRATASE | 0 |
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| BIOGENIC CATALYST FOR DELAYING PLANT DEVELOPMENT PROCESSES | 2008 |
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RU2482681C2 |
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