FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology. There are presented versions of improved nitrile hydratase, where nitrile hydratase is obtained from Rhodococcus bacteria or Nocardia bacteria. Disclosed is a DNA coding said improved nitrile hydratase, as well as an expression vector containing said DNA. Disclosed is a transformant, which is a bacterium, for producing nitrile hydratase containing said vector. Disclosed is a method of producing nitrile hydratase using said transformant. Disclosed is a method of producing an amide compound using said improved nitrile hydratase or a culture obtained by culturing said transformant, or a processed culture product.
EFFECT: group of inventions enables to obtain improved nitrile hydratase with high resistance to amide compounds at high temperatures.
12 cl, 3 dwg, 14 tbl, 9 ex
| Title | Year | Author | Number |
|---|---|---|---|
| NITRILE HYDRASE FROM Rhodococcus | 2005 |
|
RU2385932C2 |
| DNA FRAGMENT ENCODING POLYPEPTIDE WITH ACTIVITY OF NITRILE HYDRATASE, RECOMBINANT PLASMID DNA PNHJ20L ENCODING POLYPEPTIDE, STRAIN ESCHERICHIA COLI TG1/PNHJ20L, METHOD OF PREPARING POLYPEPTIDE WITH NITRILE HYDRATASE PROPERTY | 1991 |
|
RU2173708C2 |
| MUTANT NITRILE HYDRATASE, NUCLEIC ACID WHICH ENCODES SAID MUTANT NITRILE HYDRATASE, EXPRESSION VECTOR AND TRANSFORMANT CONTAINING SAID NUCLEIC ACID, METHOD OF PRODUCING SAID MUTANT NITRILE HYDRATASE AND METHOD OF PRODUCING AMIDE COMPOUND | 2017 |
|
RU2736086C1 |
| METHOD FOR PRODUCING L-AMINO ACIDS WITH USE OF BACTERIA OF ENTEROBACTERIACEAE FAMILY | 2010 |
|
RU2460793C2 |
| CYANIDE-RESISTANT NITRILE HYDRATASES | 2005 |
|
RU2385876C2 |
| METHOD FOR PREPARING DIPEPTIDE (VARIANTS) | 2005 |
|
RU2316596C2 |
| METHOD OF PREPARING L-LYSINE (VARIANTS) AND RECOMBINANT DNA USED FOR ITS REALIZATION (VARIANTS) | 1996 |
|
RU2197528C2 |
| SMALL SUBUNIT OF ISOZYME III AND ISOZYME III OF ACETOHYDROXYACID SYNTHETASE FROM ESCHERICHIA COLI, DNA FRAGMENT (VARIANTS), STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF L-VALINE (VARIANTS) AND METHOD FOR PREPARING L-VALINE | 2000 |
|
RU2209246C2 |
| Siglec-15 ANTIBODY | 2010 |
|
RU2539790C2 |
| METHOD FOR PRODUCTION OF L-AMINO ACID | 2000 |
|
RU2250266C2 |
