METHOD OF PRODUCING DNA PRIMERS AND PROBES FOR MINIMALLY INVASIVE PRENATAL PCR DIAGNOSIS OF TRISOMY OF 21ST CHROMOSOME OF FETUS IN BLOOD OF PREGNANT WOMAN AND DIAGNOSTIC KIT THEREFOR Russian patent published in 2016 - IPC G01N33/52 C12Q1/68 C12P19/34 

FIELD: medicine.

SUBSTANCE: present group of inventions relates to medicine. Disclosed is a method of obtaining DNA primers and probes for minimally invasive prenatal PCR diagnosis of trisomy of 21st chromosome in foetus in pregnant woman's blood, characterised by that it comprises selecting a site of differential methylation of foetal DNA 21st chromosome and DNA 21st chromosome of adult human, sensitive to endonucleases, synthesing direct and reverse primer corresponding to amplicon of length from 60 to 300 bps, as well as a probe which corresponds to said amplicon, performing real-time PCR of mixture of samples after restriction endonuclease processing thereof, selecting pair of primers and probes, providing efficiency of real-time PCR reaction higher than 90 % and linearity at variation of relative sample concentration higher than 90 %. Invention also discloses a kit and a minimally invasive method of prenatal PCR diagnosis in real time of trisomy of 21st chromosome in foetus in pregnant woman's blood.

EFFECT: present group of inventions provides effective agents and methods of determining trisomy of chromosome 21 of foetus in pregnant woman's blood.

16 cl, 15 dwg, 3 tbl, 1 ex