PROTEIN PURIFICATION USING BIS-TRIS BUFFER Russian patent published in 2016 - IPC C07K1/36 C07K1/22 

FIELD: chemistry.

SUBSTANCE: invention relates to a two-stage method of chromatography for purifying proteins, particularly monoclonal antibodies, using only four buffer solutions obtained from the mother solution. Method involves (a) first chromatographic stage including: passage of the said solution through the first chromatographic column; elution of crude protein from the first chromatographic column using the first elution buffer; and (b) second chromatographic stage including: passage of eluent of the crude protein obtained at the end of stage (a) through the second chromatographic column; extraction of a purified protein from the second chromatographic column using the second elution buffer, herewith each of the buffers contains Bis-Tris, acetic acid and water.

EFFECT: method enables fast production of batches of purified protein and reduction the time of using cleaning systems, that enables industrial purification of recombinant proteins.

22 cl, 6 dwg, 3 tbl, 7 ex