FIELD: biotechnology.
SUBSTANCE: present invention relates to biotechnology. Methods of purification of monoclonal antibody are disclosed. Methods involve treatment of sample with help of absorbing resin for affinity chromatography, deactivation of viruses in obtained eluate by reducing pH to 3–4, treatment of obtained eluate by means of deep-type filter with subsequent treatment by means of ion-exchange membrane and additional stage of chromatography of prepared eluate to produce monoclonal antibody. Disclosed method can include step for clarification of sample before processing on absorbing resin for affinity chromatography and stage of nanofiltration, ultrafiltration and diafiltration at final stage of purification of monoclonal antibody.
EFFECT: proposed method of purification provides high degree of purity of monoclonal antibodies without detriment to output of product and can be used for producing monoclonal antibodies with high purity.
31 cl, 8 dwg, 16 tbl, 7 ex
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