METHOD FOR PREPARING ANTIBODIES HAVING IMPROVED PROPERTIES Russian patent published in 2016 - IPC C07K16/00 A61K39/395 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and represents a Fc-containing polypeptide containing amino acid sequence SEQ ID NO: 18, where SEQ ID NO: 18 comprises mutations at amino acid positions 243 and 264 of Fc region, where Fc-containing polypeptide represents an antibody or antibody fragment containing sialylated N-glycans, and where mutation in position 243 represents F243A and mutation in position 264 represents V264A, where numbering is made according to EU index as in Kabat, where Fc-containing polypeptide has low binding with FcγRI, FcγRIIa, FcγRIIb and/or FcγRIIIa compared to parent Fc-containing polypeptide. Invention also relates to a method of producing said Fc-containing polypeptide in a host cell, as well as to a method of treating an inflammatory condition in an individual in need thereof, involving administering a therapeutically effective amount of said Fc-containing polypeptide.

EFFECT: invention enables to obtain Fc-containing polypeptide having reduced binding with FcγRI, FcγRIIa, FcγRIIb and/or FcγRIIIa compared with parent Fc-containing polypeptide.

13 cl, 22 dwg, 14 tbl, 24 ex