FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry, in particular to a genetically modified mammalian host cell, which contains a first nucleic acid coding exogenous fucosidase, and a second nucleic acid encoding endoglycosidase, as well as a method for production thereof. Modified mammalian host cell produces glycoproteins with modified glycosylation. Also disclosed is a method of producing defucosylated glycoprotein comprising using said cell.
EFFECT: invention enables effective production of defucosylated glycoprotein.
20 cl, 4 dwg
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