HYPERGLYCOSYLATED BINDING POLYPEPTIDES Russian patent published in 2019 - IPC C07K16/28 A61K38/17 A61K39/00 C12N5/10 C12N15/11 

FIELD: biochemistry.

SUBSTANCE: invention relates to biochemistry. Described is an isolated antibody or antigen-binding fragment thereof for delivering a cytotoxin comprising a CH1 IgG domain, where the CH1 IgG domain contains an asparagine residue in the amino acid position 114 in accordance with the Kabat numbering, where the side chain of the said asparagine residue is linked to the glycan. Also described is an isolated antibody or its antigen-binding fragment for the delivery of a detection agent containing a CH1 IgG domain, where the CH1 domain of the IgG contains an asparagine residue in the amino acid position 114 in accordance with the Kabat numbering, where the side chain of the said asparagine residue is linked to the glycan. Also described is an isolated antibody or its antigen-binding fragment for the delivery of a cytotoxin containing a CH1 domain of IgG, where the CH1 domain of the IgG contains an asparagine residue in the amino acid position 114 in accordance with the Kabat numbering, where the side chain of said asparagine residue is bound to glycan by β-glycosylamide linkage and where glycan is linked to detection agent. Also described is an isolated antibody or antigen-binding fragment thereof for the delivery of a detection agent containing a CH1 IgG domain, where the CH1 IgG domain contains the asparagine residue in the amino acid position 114 in accordance with the Kabat numbering, where the side chain of said asparagine residue is bound to glycan by β-glycosylamide linkage and where glycan is linked to cytotoxin. Also disclosed is an isolated antibody or an antigen-binding fragment thereof for cytotoxin binding, containing an CH1 domain of IgG, wherein the CH1 domain of IgG contains a free asparagine residue in the position of amino acid 114 in accordance with the Kabat numbering. Disclosed is an isolated antibody or antigen-binding fragment thereof for binding a detection agent containing a CH1 IgG domain, wherein the CH1 domain of the IgG contains a free asparagine residue at amino acid position 114 in accordance with the Kabat numbering.

EFFECT: invention can be used for treating cancer, for diagnostic use.

36 cl, 35 dwg, 15 tbl, 15 ex