RECOMBINANT PLASMID DNA pEFN877, DETERMINING EXPRESSION OF THE HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE SURFACE OF ESCHERICHIA COLI CELLS, AND STRAIN OF BACTERIA ESCHERICHIA coli BL21(DE3)pLysS/pEFN877 - PRODUCER OF HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE CELLS SURFACE Russian patent published in 2017 - IPC C12N15/12 C12N15/28 C12N15/62 C12N15/63 C12N1/21 C12R1/19 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and can be used for producing the hybrid polypeptide alternative carcass protein 10-th human fibronectin domain. Recombinant plasmid DNA pEFN877 designed on the basis of plasmid pET20b(+) length 3.654 t.p.o. Obtained plasmid pEFN877 comprises hybrid of gene EFN877 with SEQ ID NO: 8, coding amino acid sequences of esterase P. cryohalolentis K5^T, 10-th domain of human fibronectin and autotransporter P. cryohalolentis K5^T in a single reading frame, arranged in accordance with fig. 1. Bacterial strain E. coli BL21(DE3)pLysS/pEFN877 is obtained by transformation of competent cells E. coli BL21(DE3)pLysS of plasmid DNA pEFN877.

EFFECT: invention allows to obtain cell strain E.coli – producer of hybrid polypeptide with properties of 10-th human fibronectin domain on the cells surface.

2 cl, 4 dwg, 9 ex