FIELD: biotechnologies.
SUBSTANCE: recombinant DNA is produced, which codes functionally active hybrid protein (BrdGl7ACA-cbd), consisting of amino-acid sequence of acylase glutaryl-7- aminocephalosporanic acid of strain Brevundimonas diminuta All-Russian collection of industrial microorganisms B-1297 and chitin-binding domain of chitinase Al Bacillus circulans. Recombinant plasmid pSVH0108 is constructed for expression of BrdGl7ACA-cbd in cells E.coli, containing sequence of recombinant DNA that codes hybrid protein under control of promotor and terminator of RNA-polymerase of phage T7. As a result of E.coli strain tranformation with this recombinant plasmid and selection of transformed clones, a new strain E.coli BL21(DE3)/pSVH0108 cbd is produced - producer of hybrid protein BrdGl7ACA-cbd.
EFFECT: high yield of recombinant ferment.
4 dwg, 2 tbl, 7 ex
| Title |
Year |
Author |
Number |
| MODIFIED RAS GENE OF ESCHERICHIA COLI BACTERIUM, CODING PRECURSOR OF ENZYME WITH PENICILLIN G ACYLASE ACTIVITY, RECOMBINANT ESCHERICHIA COLI STRAIN - PRODUCER OF ACYLASE PENICILLIN G AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF THIS ENZYME |
2015 |
- Eldarov Mikhail Anatolevich
- Sklyarenko Anna Vladimirovna
- Dumina Mariya Vladimirovna
- Satarova Dzhenni Ernstovna
- Zhgun Aleksandr Aleksandrovich
- Medvedeva Natalya Viktorovna
- Yarotskij Sergej Viktorovich
|
RU2624022C1 |
| RECOMBINANT PLASMID pSVH0106 PROVIDING SYNTHESIS OF Gl7ACA ACYLASE IN Escherichia coli CELLS, RECOMBINANT STRAIN OF Escherichia coli BL21(DE3)/pSVH0106 AS PRODUCER OF Gl7ACA ACYLASE |
2005 |
- Khatuntseva Svetlana Anatol'Evna
- Ehl'Darov Mikhail Anatol'Evich
- Zejnalov Orkhan Akhmed Ogly
- Skrjabin Konstantin Georgievich
|
RU2300566C2 |
| RECOMBINANT DNA ENCODING FUNCTIONALLY ACTIVE HYBRID PROTEIN OF D-AMINO ACID OXYDASE WITH CHITIN-COUPLING DOMAIN (DAOcbd), pVR1 RECOMBINANT PLASMID PROVIDING SYNTHESIS THEREOF IN Escherichia coli CELLS AND RECOMBINANT Escherichia coli STRAIN C 41 (DE3)/pVR1 AS PRODUCER OF DAOcbd |
2006 |
- Redo Vadim Aleksandrovich
- Ehl'Darov Mikhail Anatol'Evich
- Khatuntseva Svetlana Anatol'Evna
- Zhgun Aleksandr Aleksandrovich
- Zejnalov Orkhan Akhmed Ogly
- Skrjabin Konstantin Georgievich
|
RU2310688C1 |
| pETTvDAO2 RECOMBINANT PLASMID PROVIDING SYNTHESIS OF YEAST Trigonopsis variabilis D-AMINO ACID OXYDASE (DAO) IN Escherichia coli CELLS AND RECOMBINANT Escherichia coli STRAIN C41(DE3)/pETTvDAO2 AS PRODUCER OF DAO |
2006 |
- Ehl'Darov Mikhail Anatol'Evich
- Redo Vadim Aleksandrovich
- Zhgun Aleksandr Aleksandrovich
- Zejnalov Orkhan Akhmed Ogly
- Skrjabin Konstantin Georgievich
|
RU2310687C1 |
| RECOMBINANT PLASMID DNA CODING FUSION PROTEIN L-HEP, ESCHERICHIA COLI STRAIN PRODUCER OF SAID PROTEIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN |
2019 |
- Myagkikh Igor Valentinovich
- Stepanenko Vasilij Nikolaevich
- Makarov Dmitrij Aleksandrovich
- Sokolova Irina Vladimirovna
- Vorobeva Tatyana Vladimirovna
- Levandovskaya Kristina Georgievna
- Sveshnikova Elena Vasilevna
- Zinchenko Aleksej Alekseevich
- Pavlenko Daniil Mikhajlovich
- Nokel Elena Adamovna
- Melikhova Tatyana Dmitrievna
- Dolgikh Dmitrij Aleksandrovich
- Gasparyan Marine Eduardovna
- Miroshnikov Anatolij Ivanovich
- Korolev Ivan Romanovich
- Kalinin Danil Sergeevich
- Puchkov Ilya Aleksandrovich
- Novozhilov Nikolaj Mikhajlovich
- Kirpichnikov Mikhail Petrovich
|
RU2716975C1 |
| RECOMBINANT PLASMID PROVIDING SYNTHESIS OF BARNASE IN CELLS OF ESCHERICHIA COLI, ESCHERICHIA COLI STRAIN - PRODUCER OF BARNASE AND METHOD FOR PRODUCING BARNASE |
2017 |
- Deev Sergej Mikhajlovich
- Shulga Aleksej Anatolevich
- Lukyanova Tamara Ivanovna
- Konovalova Elena Valerevna
|
RU2650871C1 |
| RECOMBINANT PLASMID DNA ENCODING HYBRID POLYPEPTIDES WITH PROPERTIES OF RED FLUORESCENT PROTEIN mCherry TO PRODUCE HYBRID FLUORESCENT PROTEINS IN Escherichia coli |
2013 |
- Petrovskaja Lada Evgen'Evna
- Shingarova Ljudmila Nikolaevna
- Gapizov Sultan Shakhbanovich
- Krjukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Jakimov Sergej Aleksandrovich
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2527171C1 |
| ARTIFICIAL GENE, CODING CIMERIC PROTEIN OF HUMAN ANGIOGENIN, CHIMERIC PLASMID pJZZ-A, ENSURING EXPRESSION OF GENE OF CHIMERIC PROTEIN OF HUMAN ANGIOGENIN IN Escherichia coli AND STRAIN OF Escherichia coli BL21(DE3)/pJZZ-A-PRODUCENT OF RECOMBINANT CHIMERIC PROTEIN OF HUMAN ANGIOGENIN |
2012 |
- Ramazanov Jurij Akhmetovich
- Beklemishev Anatolij Borisovich
|
RU2512527C2 |
| RECOMBINANT DNA, CODING HUMAN GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) AND RECOMBINANT PLASMID pAS017, PROVIDING G-CSF SYNTHESIS IN CELLS OF Escherichia coli |
2011 |
- Shul'Ga Aleksej Anatol'Evich
|
RU2529363C2 |
| RECOMBINANT PLASMID DNA pET32M/mTrx-rhIL-11 CODING HUMAN INTERLEUKIN-11, METHOD OF PRODUCING AND Escherichia coli BL21 (DE3)/pET32M/mTrx-rhIL-11 STRAIN - PRODUCER OF RECOMBINANT INTERLEUKIN-11 |
2009 |
- Miroshnikov Anatolij Ivanovich
- Vorob'Ev Ivan Ivanovich
- Ponomarenko Natal'Ja Aleksandrovna
- Smirnov Ivan Vital'Evich
|
RU2426787C2 |
