RECOMBINANT PLASMID DNA pEstPc, ENCODING POLYPEPTIDE WITH PROPERTIES OF ESTERASE Psychrobacter cryohalolentis K5, Escherichia coli BACTERIA STRAIN - PRODUCER OF POLYPEPTIDE WITH PROPERTIES OF ESTERASE Psychrobacter cryohalolentis K5 Russian patent published in 2013 - IPC C12N15/63 C12N15/64 C12N15/66 C12N15/72 C12N1/20 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Described is a plasmid which codes esterase Psychrobacter cryohalolentis K5^T and contains Ndel/Xhol - a DNA fragment of plasmid pET32a with length of 5.366 thousand base pairs, which includes a promoter of bacteriophage T7, a translation enhancer of gene 10 of the bacteriophage T7, a transcription terminator of the bacteriophage T7, a bla β-lactamase gene which determines resistance of cells transformed by plasmid pPC023 to ampicillin, a replication initiation section ori; and Ndel/Sall - a DNA fragment with size of 0.869 thousand base pairs, which contains a gene which codes the mature form of esterase Psychrobacter cryohalolentis K5^T with an amino acid sequence SEQ ID NO: 2, given in the description, having an C-end hexahestidine linker. Provided is an Escherichia coli bacteria strain which is modified by said plasmid, a producer of a polypeptide having esterase Psychrobacter cryohalolentis K5^T activity.

EFFECT: invention increases the level of biosynthesis of esterase to 20% of total cell protein.

2 cl, 2 dwg, 5 ex