FIELD: biotechnology.
SUBSTANCE: process for detergent-free preparation of outer membrane vesicles (OMV) of Gram negative Neisseria bacteria is disclosed. Method involves culturing bacteria population up to the stage of stationary growth followed by incubation in a medium with pH higher than 8.0 and concentration of an metalchelated agent of 1–100 mm and extracting abstracted OMV.
EFFECT: proposed method ensures high output and purity of produced OMV and can be used in biotechnology in large-scale production of vaccines, containing outer membrane vesicles (OMV) of Gram negative Neisseria bacteria.
12 cl, 2 dwg, 4 tbl, 1 ex
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