FIELD: cosmetics.
SUBSTANCE: invention refers to cosmetic and pharmaceutical industry and represents a method for producing a cosmetic composition of fluid formed during the hatching of fish roe, including at least the following steps: (a) suspending of fish roe in minimal volume of water; (b) induction of synchronized fast hatching said caviar, preferably so, that hatching is terminated for less than 6 hours for more than 80% of embryos; (c) filtration of liquid, formed at hatching, from step (b) to obtain a composition, note here that said fluid filtration stage, formed at hatching includes a liquid filter formed at hatching, using a filter with pore size of at least 5 mcm and collecting of the filtrate; (d) treating the filtrate from stage (c) by means of ion-exchange chromatography, including: (1) loading of the filtrate to ion-exchange column, preferably on DEAE (diethylaminoethylic) column; (2) washing the column by suitable buffer, preferably buffered by irrigating solution with pH 7–9; (3) elution of column leucolectin polypeptides using first elution buffer or solvent, note here that preferably the first elution buffer comprises buffered flushing solution, additionally contains salt in concentration of 50 to 100 mM; (4) elution from column of remaining polypeptides using second elution buffer or solvent, at that, preferably second elution buffer comprises buffered flushing solution, containing salt in concentration of 500 mM to 2 M; (5) collecting eluate from step (4); (e) replacement of water in the eluate from step (5) on a cosmetically acceptable buffer; (f) filtering the solution obtained at step (e), using a filter with pore size of 0.15–0.30 mcm and collecting the filtrate; and (g) preparation of said cosmetic composition from the filtrate obtained at step (f).
EFFECT: invention provides effective skin moistening and pilling of the stratum corneum.
25 cl, 7 dwg, 1 tbl, 6 ex
