FIELD: bioengineering.
SUBSTANCE: polyclonal or monoclonal antibody is suggested, it specifically links epitope out of TKSLDKGYNK peptides of Clostridium neurotoxins, and is produced by use of oligopeptide with said epitope. Method of production of antibody specific for proteolytic unprocessed and/or partially processed botulinic neurotoxins type A. Polyclonal protective serum produced by immunization by TKSLDKGYNKA immunogen is mixed with said peptide. The produced complex of peptide and antibodies are removed from protective serum. The antibody is separated from the said complex. Polyclonal antibody produced by said method and producing complex with TKSLDKGYNKA is described. Use of antibodies is suggested for separation or determination of proteolytically partially processed and/or unprocessed polypeptide. Method of production of proteolytically processed polypeptide of botulinic neurotoxins type A is suggested. Solution with mixture of proteolytically processed, partially processed and/or unprocessed polypeptide botulinic neurotoxins type A is mixed with antibody with creation of complexes of antibodies with proteolytically partially processed and/or unprocessed polypeptide. The produced complex of peptide and antibody is removed from solution. Method of production of medicament is described, under it the method of production of proteolytically processed polypeptide botulinic neurotoxins type A is used with further formation of said solution as the medicament.
EFFECT: separation of proteolytically partially processed or unprocessed polypeptide botulinic neurotoxins type A, this can be used to produce the medicinal compositions of botulinic neurotoxins type A.
16 cl, 3 dwg, 2 tbl, 2 ex
