METHOD OF PRODUCING PROBIOTIC COMPOSITION Russian patent published in 2017 - IPC C12N1/20 A23C9/12 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, food and medical industry and can be used in production of probiotics for direct consumption or biologically active additive to food products, in production of fermented milk products of preventive purpose, intended particularly for normalization of cholesterol level, and useful microflora of gastrointestinal tract and increasing general body resistance. Invention involves method of producing probiotic composition for lowering of blood cholesterol, containing dry biomass of probiotic bacteria strains B. bifidum GG-72 VKPM Ac-1884, L. fermentum LFM-2 VKPM V-10368, L. rhamnosus LC-52GV VKPM V-9475, L. plantarum GVI-1 VKPM V-8556, L. acidophilus AST-44. Above method involves separate cultivation of strains B. bifidum GG-72 VKPM Ac-1884 on Blaurock medium, strain L. fermentum LFM-2 VKPM V-10368, L. rhamnosus LC-52GV VKPM V-9475, L. plantarum GVI-1 VKPM V-8556 and L. acidophilus ACT-44 B. bifidum GG-72 VKPM Ac-1884, L. fermentum LFM-2 VKPM V-10368, L. rhamnosus LC-52GV VKPM V-9475, L. plantarum GVI-1 VKPM V-8556, L. acidophilus AST-44 VKPM-V-9647 on MRS broth at 37±1 °C up to stationary phase of development, concentration, mixing with protective medium, drying and mixing of dry biomass of probiotic strains in equal quantities.

EFFECT: method of producing probiotic composition is disclosed.

2 cl, 8 dwg, 6 tbl