FIELD: biotechnology.
SUBSTANCE: invention relates to microbiology and biotechnology, namely to hybridoma technology. Invention represents cultivated hybrid H-24 cell clone producing in environment of cell culture and abdominal cavity of syngeneic animals monoclonal antibody (MA) H-24 to SEH. Clone is produced by fusion of cells of mouse myeloma SP-2/0 with popliteal lymph node cells of BALB/c mice immunized with SEH subcutaneously. Fusing agent is polyethylene glycol with molecular weight of 1,500. Hybridoma selection has been performed on medium DMEM with bovine foetal serum and addition of hypoxantine-aminopterin-thymidine. Hybridoma synthesizes MA, specifically interacting with SEH. Antibody titre in culture liquid reaches no less than 1:10,000 1:20,000, in ascitic one it is no less than 1:1,000,000. Antibodies can be used for designing immunochemical SEH detection systems. In version of sandwich ELISA using biotinylated MA detection of H-18 and streptavidin peroxidase SEH is detected in concentration range from 0.2 (minimum limit of determination) up to 3 ng/ml.
EFFECT: invention can be used for detection of staphylococcal enterotoxin H (SEH).
1 cl, 3 dwg, 1 tbl, 7 ex
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