ANIMAL MUS MUSCULUS HYBRID CELL F3H10 CLONE L-PRODUCER OF MONOCLONAL DIPHTHERIA TOXIN ANTIBODIES Russian patent published in 2010 - IPC C12N15/18 C12P21/08 

FIELD: medicine.

SUBSTANCE: invention concerns a new F3H10 hybrid cell clone producing monoclonal diphtheria toxin antibody in the environment of cell culture and abdominal cavity of syngeneic animals. The clone is produced by the fusion of mouse myeloma SP-2/0 with popliteal lymph node cells of BALB/c mice immunised with a preparation of diphtheria toxin (manufactured by State Institution Gamaleya's Epidemiology and Microbiology Research Institute) in posterior pads. A fusing agent is polyethylene glycol of molecular weight 4000. Hybridoma selection has been performed on Dulbecco modified Eagle's medium with bovine foetal serum and hypoxantine-aminopterin-thymidine added. Hybridoma synthesises monoclonal antibodies specifically reacting with diphtheria toxin and belonging to the IgG class as determined by IEA data with typifying antiserums against certain murine Ig classes. Antibody titre reaches in the culture fluid 1:10000 1:20000, and in the ascitic 1:1000000.

EFFECT: antibodies can be used for designing immunobiological test systems of diphtheria toxin detection exceeding available analogues in sensitivity and reproducibility.

2 dwg, 4 ex