ANIMAL MUS MUSCULUS HYBRID CELL CLONE L-PRODUCER OF MONOCLONAL CHOLERA TOXIN ANTIBODIES Russian patent published in 2010 - IPC C12N5/00 

Abstract RU 2401299 C1

FIELD: medicine.

SUBSTANCE: there is produced a new CT-B1/F8 hybrid cell clone producing monoclonal cholera toxin antibody (MCAB) in the environment of cell culture and abdominal cavity of syngeneic animals. The clone is produced by the fusion of mouse myeloma SP-2/0 cells with popliteal lymph node cells of BALB/c mice immunised with a commercial preparation of cholera toxin (SIGMA) in posterior pads. A fusing agent is polyethylene glycol of molecular weight 4000. Hybridoma selection has been performed on Dulbecco modified Eagle's medium with bovine foetal serum and hypoxantine-aminopterin-thymidine added. Hybridoma synthesises MCAB specifically interacting with cholera toxin and not interacting with thermolabile E.coli toxin. The antibody titre reaches 1:20000 in the cultural fluid, 1:2000000 in ascitic.

EFFECT: antibodies can be used for designing immunobiological systems of cholera toxin detection exceeding available analogues in sensitivity.

2 dwg, 4 ex

Similar patents RU2401299C1

Title Year Author Number
ANIMAL MUS MUSCULUS HYBRID CELL CLONE L-PRODUCER OF MONOCLONAL CHOLERA TOXIN ANTIBODIES 2009
  • Valjakina Tat'Jana Ivanovna
  • Grishin Evgenij Vasil'Evich
  • Petrova Elena Ehduardovna
  • Komaleva Ravilja Ljukmanovna
RU2401301C1
ANIMAL MUS MUSCULUS HYBRID CELL CLONE L-PRODUCER OF MONOCLONAL CHOLERA TOXIN ANTIBODIES 2009
  • Valjakina Tat'Jana Ivanovna
  • Grishin Evgenij Vasil'Evich
  • Petrova Elena Ehduardovna
  • Komaleva Ravilja Ljukmanovna
RU2401300C1
ANIMAL MUS MUSCULUS HYBRID CELL F3H10 CLONE L-PRODUCER OF MONOCLONAL DIPHTHERIA TOXIN ANTIBODIES 2009
  • Valjakina Tat'Jana Ivanovna
  • Lakhtina Ol'Ga Evgen'Evna
  • Komaleva Ravilja Ljukmanovna
  • Grishin Evgenij Vasil'Evich
RU2407795C1
ANIMAL MUS MUSCULUS HYBRID CELL G10B6 CLONE L-PRODUCER OF MONOCLONAL DIPHTHERIA TOXIN ANTIBODIES 2009
  • Valjakina Tat'Jana Ivanovna
  • Lakhtina Ol'Ga Evgen'Evna
  • Komaleva Ravilja Ljukmanovna
  • Grishin Evgenij Vasil'Evich
RU2401303C1
HYBRID STRAIN OF CULTURED CELLS OF ANIMALS MUS MUSCULUS -T 2E5 - PRODUCER OF MONOCLONAL ANTIBODIES ISOTYPE G 1 TO B SUBUNIT OF CHOLERA TOXIN 2015
  • Mikheeva Elena Aleksandrovna
  • Devdariani Zurab Levanovich
  • Osina Natalija Aleksandrovna
RU2583306C1
CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS HT 3E5 - PRODUCER OF MONOCLONAL ANTIBODIES ISOTYPE G 2A TO B- SUBUNIT OF CHOLERA TOXIN 2015
  • Mikheeva Elena Aleksandrovna
  • Devdariani Zurab Levanovich
  • Osina Nataliya Aleksandrovna
RU2590587C1
STRAIN OF HYBRID CULTURED CELLS MUS MUSCULUS 365E11 PRODUCING MONOCLONAL ANTIBODIES TO SHIGA-LIKE TOXIN TYPE II 2020
  • Kuklina Galina Viktorovna
  • Pechenkin Denis Valerievich
  • Eremkin Andrej Valentinovich
  • Kytmanov Aleksej Aleksandrovich
  • Ipatov Sergej Sergeevich
  • Gorshkov Anton Sergeevich
RU2768838C1
CLONE OF CULTIVATED HYBRID ANIMAL CELLS MUS MUSCULUS L.H-24-PRODUCER OF MONOCLONAL ANTIBODIES SPECIFIC TO STAPHYLOCOCCAL ENTEROTOXIN H (SEH) 2016
  • Rudenko Natalya Vasilevna
  • Karatovskaya Anna Petrovna
  • Guseva Kseniya Aleksandrovna
  • Shepelyakovskaya Anna Olegovna
  • Laman Aleksandr Georgievich
  • Loskutova Irina Vladimirovna
  • Boziev Khanafij Magometovich
  • Brovko Fedor Aleksandrovich
RU2616289C2
METHOD AND SET FOR DETERMINATION OF CHOLERA TOXIN PRODUCTION AND DIFFERENTIATION OF EPIDEMIOLOGICALLY SIGNIFICANT STRAINS OF CHOLERA VIBRIOS OF CLASSICAL AND EL TOR BIOVARS 2016
  • Mikheeva Elena Aleksandrovna
  • Osina Nataliya Aleksandrovna
  • Devdariani Zurab Levanovich
  • Shcherbakova Svetlana Anatolevna
  • Kutyrev Vladimir Viktorovich
RU2611359C1
TEST SYSTEM FOR DIAGNOSTICS OF AGENTS OF ACUTE RESPIRATORY VIRAL INFECTIONS 2019
  • Klotchenko Sergei Anatolevich
  • Vasin Andrei Vladimirovich
  • Plotnikova Marina Aleksandrovna
  • Taraskin Aleksandr Sergeevich
  • Lozhkov Aleksei Aleksandrovich
  • Giulikhandanova Nataliia Evgenevna
  • Elpaeva Ekaterina Sergeevna
  • Griadunov Dmitrii Aleksandrovich
  • Filippova Marina Aleksandrovna
  • Savvateeva Elena Nikolaevna
RU2733379C1

RU 2 401 299 C1

Authors

Valjakina Tat'Jana Ivanovna

Grishin Evgenij Vasil'Evich

Petrova Elena Ehduardovna

Komaleva Ravilja Ljukmanovna

Dates

2010-10-10Published

2009-04-06Filed