ANIMAL MUS MUSCULUS HYBRID CELL G10B6 CLONE L-PRODUCER OF MONOCLONAL DIPHTHERIA TOXIN ANTIBODIES Russian patent published in 2010 - IPC C12N15/00 

FIELD: medicine.

SUBSTANCE: there is presented a new G10B6 hybrid cell clone producing monoclonal diphtheria toxin antibody in the environment of cell culture and abdominal cavity of syngeneic animals. The clone is produced by the fusion of mouse myeloma SP-2/0 cells with popliteal lymph node cells of BALB/c mice immunised with a commercial preparation of diphtheria toxin (manufactured by State Institution Gamaleya's Epidemiology and Microbiology Research Institure) in posterior pads. A fusing agent is polyethylene glycol of molecular weight 4000. Hybridoma selection has been performed on Dulbecco modified Eagle's medium with bovine foetal serum and hypoxantine-aminopterin-thymidine added. Hybridoma synthesizes monoclonal antibodies specifically reacting with diphtheria toxin. The antibody titre is 1:10000 1:20000 in the culture fluid, and 1:1000000 in ascitic. Antibodies can be used for designing immunobiological systems of diphtheria toxin detection exceeding available analogues in sensitivity, specificity and reproducibility.

EFFECT: invention can be used for creation of the diagnostic test-systems exceeding available analogues in sensitivity, specificity and reproducibility.

2 dwg, 4 ex