FIELD: biotechnology.
SUBSTANCE: pseudotyped viral vector particle for biological material transfer into hematopoietic cells, a method for its preparation and application, a drug for hematopoietic disorders or autoimmune diseases treatment, comprising said particles, a method for hematopoietic cells transducing and a stable virus packing cell line are proposed. The proposed pseudotyped viral vector particle is lentiviral and contains a chimeric enveloped glycoprotein or a modified envelope glycoprotein of baboon endogenous retrovirus (BaEV). This chimeric enveloped glycoprotein contains a transmembrane domain and an extracellular envelope BaEV glycoprotein and cytoplasmic tail domain of the enveloped glycoprotein of the murine leukemia virus (MLV). The modified enveloped BaEV glycoprotein does not contain the fusion inhibiting R peptide in the cytoplasmic tail domain.
EFFECT: proposed vector particle efficiently transduces hematopoietic cells and can be used in genetic therapy.
13 cl, 27 dwg, 2 tbl, 1 ex
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