FIELD: bioengineering.
SUBSTANCE: suggested system of the stable expression of lentivirus proteins comprises the hybrid vector containing a baculovirus framework, in its turn containing an integration casette, the flanked ITR AAV, containing two expression cassettes, and the expressing plasmid containing an open reading frame (ORF) Rep AAV. Wherein the first cassette encodes the lentivirus genes gag and pol, and second cassette encodes the rev and a selected marker. The system ensures the production of the line of cells containing structural and regulatory proteins HIV gag/pol and rev used to produce LV. The inventions can be used in gene therapy.
EFFECT: increased stability.
19 cl, 3 dwg, 4 tbl, 3 ex
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