STABLE PRODUCTION OF LENTIVIRUS VECTORS Russian patent published in 2016 - IPC C12N15/867 C12N7/01 C12N5/10 

FIELD: bioengineering.

SUBSTANCE: inventions relate to the methods of production of the stable line of lentiviruses packing cells (versions), the stable line of the lentiviruses packing cells, the method of producing the lentivirus vectors, the line of cell-producers, and the method of producing the lentivirus vectors. The suggested inventions are made using the hybrid system of baculo-AAV for the stable expression of structural and regulatory lentivirus proteins. Such a system contains the baculovirus framework, containing an integration cassette, flanked ITR AAV, in a combination with the plasmid coding a rep protein. The suggested diagram ensures the stable integration of the structural and regulatory proteins HIV-1 gag/pol and rev, and production of the first intermediate product containing only structural and regulatory proteins HIV gag/pol and rev, used as the input point to produce the stable lines of the packing cells, as well as the lines of cells-producers. The inventions can be used in gene therapy.

EFFECT: increased stability.

30 cl, 6 dwg, 6 tbl, 6 ex