COMPOSITIONS AND METHOD FOR CHROMOSOMAL ABERRATIONS DETERMINATION WITH NEW HYBRIDIZATION BUFFERS Russian patent published in 2017 - IPC C12Q1/68

Abstract RU 2618868 C2

FIELD: medicine, pharmacy.

SUBSTANCE: invention refers to biotechnology, namely to the method for determination of chromosomal aberrations presence in the DNA, and can be used in medicine. Cyclic aprotic solvent usage in the proposed method provides reliable detection of signal at lower denaturation temperatures compared to conventional compositions for hybridization.

EFFECT: invention eliminates application of formamide or reduces the dependence on formamide during hybridization of double-stranded nucleic acid sequences.

28 cl, 2 dwg, 3 tbl, 22 ex

Similar patents RU2618868C2

Title	Year	Author	Number
COMPOSITIONS AND METHODS FOR HYBRIDISATION	2009	Khansen Charl'Z M. Mattisen Sten Khauge Petersen Kennet Kh. Poul'Sen Tim Svenstrup	RU2505609C2
METHOD FOR SYNTHESISING A FLUORESCENTLY LABELLED DNA PROBE FOR A REACTION OF FLUORESCENT IN SITU HYBRIDISATION (FISH)	2020	Lagutinskaia Daria Vladimirovna Olkhovskii Igor Alekseevich Stoliar Marina Aleksandrovna Gorbenko Aleksei Sergeevich	RU2761308C1
METHOD OF MARKING HUMAN 7TH CHROMOSOME IN METAPHASIC AND INTERPHASIC CELLS	2009	Jurov Jurij Borisovich Aleksandrov Ivan Aleksandrovich Solov'Ev Il'Ja Vladimirovich Jurov Ivan Jur'Evich Vorsanova Svetlana Grigor'Evna	RU2425890C2
CO-LOCALISED CHROMATIN TRAPPING AND CHARACTERISING	2006	De Lat Vauter Grosveld Frank	RU2478716C2
RECOMBINANT PLASMID DNA PYAI 960 DESIGNATED FOR HUMAN 18-TH CHROMOSOME MARKING	2000	Jurov Ju.B. Vorsanova S.G. Aleksandrov I.A.	RU2161199C1
RECOMBINANT PLASMID DNA PYAIA05 FOR MARKING AND IDENTIFICATION OF THE THIRD HUMAN CHROMOSOME	1994	Jurov Ju.B. Vorsanova S.G. Aleksandrov I.A.	RU2087533C1
RECOMBINANT PLASMID DNA ALPHA R 1-6 DESIGNATED FOR MARKING THE 13-TH AND 21-D HUMAN CHROMOSOMES	1995	Solov'Ev I.V. Jurov Ju.B. Vorsanova S.G. Jakovlev A.G. Demidova I.A.	RU2087534C1
RECOMBINANT PLASMID DNA pYAII-39, USED FOR 4TH AND 9TH HUMAN CHROMOSOME MARKING IN METAPHASIC AND INTERPHASIC CELLS	2006	Jurov Jurij Borisovich Aleksandrov Ivan Aleksandrovich Vorsanova Svetlana Grigor'Evna Solov'Ev Il'Ja Vladimirovich	RU2325441C1
RECOMBINANT PLASMID DNA ALPHA-R-12 DESIGNATED FOR MARKING 6-TH HUMAN CHROMOSOME IN METAPHASE IN INTERPHASE CELLS	2004	Jurov Ju.B. Jakovlev A.G. Vorsanova S.G. Solov'Ev I.V.	RU2265060C1
METHOD FOR DETECTION OF STRUCTURAL REARRANGEMENTS OF TUMOR CELLS (CHEMERIC GENES) GENOME, DETERMINING SELECTION OF THERAPY AND PREDICTION IN ACUTE LEUKOSIS IN CHILDREN, USING OT-PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP)	2016	Ikonnikova Anna Yurevna Fesenko Denis Olegovich Karateeva Anna Vladimirovna Zasedatelev Aleksandr Sergeevich Nasedkina Tatyana Vasilevna	RU2639513C1

RU 2 618 868 C2

Authors

Mattisen Sten Khauge

Petersen Kennet Kh.

Poulsen Tim Svenstrup

Dates

2017-05-11—Published

2009-05-27—Filed