FIELD: medicine, pharmacy.
SUBSTANCE: invention refers to biotechnology, namely to the method for determination of chromosomal aberrations presence in the DNA, and can be used in medicine. Cyclic aprotic solvent usage in the proposed method provides reliable detection of signal at lower denaturation temperatures compared to conventional compositions for hybridization.
EFFECT: invention eliminates application of formamide or reduces the dependence on formamide during hybridization of double-stranded nucleic acid sequences.
28 cl, 2 dwg, 3 tbl, 22 ex
| Title | Year | Author | Number |
|---|---|---|---|
| COMPOSITIONS AND METHODS FOR HYBRIDISATION | 2009 |
|
RU2505609C2 |
| METHOD FOR SYNTHESISING A FLUORESCENTLY LABELLED DNA PROBE FOR A REACTION OF FLUORESCENT IN SITU HYBRIDISATION (FISH) | 2020 |
|
RU2761308C1 |
| METHOD OF PRODUCING PANEL OF DNA PROBES FOR DETERMINING CHROMOSOMAL TRANSLOCATIONS, DELETIONS AND AMPLIFICATIONS | 2023 |
|
RU2828103C1 |
| METHOD OF MARKING HUMAN 7TH CHROMOSOME IN METAPHASIC AND INTERPHASIC CELLS | 2009 |
|
RU2425890C2 |
| CO-LOCALISED CHROMATIN TRAPPING AND CHARACTERISING | 2006 |
|
RU2478716C2 |
| RECOMBINANT PLASMID DNA PYAI 960 DESIGNATED FOR HUMAN 18-TH CHROMOSOME MARKING | 2000 |
|
RU2161199C1 |
| RECOMBINANT PLASMID DNA PYAIA05 FOR MARKING AND IDENTIFICATION OF THE THIRD HUMAN CHROMOSOME | 1994 |
|
RU2087533C1 |
| RECOMBINANT PLASMID DNA ALPHA R 1-6 DESIGNATED FOR MARKING THE 13-TH AND 21-D HUMAN CHROMOSOMES | 1995 |
|
RU2087534C1 |
| RECOMBINANT PLASMID DNA pYAII-39, USED FOR 4TH AND 9TH HUMAN CHROMOSOME MARKING IN METAPHASIC AND INTERPHASIC CELLS | 2006 |
|
RU2325441C1 |
| RECOMBINANT PLASMID DNA ALPHA-R-12 DESIGNATED FOR MARKING 6-TH HUMAN CHROMOSOME IN METAPHASE IN INTERPHASE CELLS | 2004 |
|
RU2265060C1 |
