CO-LOCALISED CHROMATIN TRAPPING AND CHARACTERISING Russian patent published in 2013 - IPC C12Q1/68 C12Q1/44 C12P19/34 C12N15/11 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to biotechnology, particularly a method for analysing a reaction frequency of a target nucleotide sequence and one or more nucleotide sequences of concern. The method provides (a) preparing a sample of cross-linked DNA and (b) cross-linked DNA cleavage by a first restriction fragment. It is followed by (c) ligation of the cross-linked nucleotide sequences, (d) removal of the cross links; (e) nucleotide sequence cleavage by a second restriction fragment, and (f) ligation of one or more DNA sequences having a known nucleotide composition with accessible cleavage site(s) by the second restriction fragment which flanks one or more nucleotide sequences of concern. Then, (g) one or more nucleotide sequences of concern are amplified with the use of two nucleotide primers with each primer hybridised with DNA sequences which flank the nucleotide sequences of concern. It is followed by (h) hybridisation of the amplified sequence(s) with a chip; and (i) determination of the reaction frequency of the DNA sequences.

EFFECT: what is presented is the method for co-localised chromatin trapping and characterising.

29 cl, 19 dwg, 2 tbl, 8 ex