FIELD: biotechnology.
SUBSTANCE: pET-15b_T1_RL plasmid DNA is constructed which provides synthesis of recombinant fusion protein consisting of a tumor-specific peptide and an antitumor peptide RL2. The recombinant fusion protein isolated from Escherichia coli cells transformed with pET-15b_T1_RL plasmid, has a molecular weight of 15.7 kDa, consists of methionine residue, tumor specific peptide GLHTSATNLYLH, glycine GGGS spacer, and a recombinant lactaptin RL2 analogue.
EFFECT: production of a protein providing cytotoxic activity against cancer cells in culture and targeted properties for tumor tissue.
2 cl, 6 dwg, 5 ex
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| ANTIGENIC COMPOSITION AND THERAPEUTIC APPLICATION THEREOF FOR PREVENTING AND TREATING ONCOLOGICAL DISEASES, RECOMBINANT PLASMID DNA, PROVIDING SYNTHESIS OF HYBRID PROTEIN, AS WELL AS METHOD OF PRODUCING PROTEIN | 2013 |
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