METHOD OF ESTIMATION OF THE APOPTOSIS PROCESS IN THE FABRIC OF THE BRAIN OF LABORATORY ANIMALS Russian patent published in 2017 - IPC G09B23/28 B82B1/00 

FIELD: medicine.

SUBSTANCE: invention concerns the criteria for the diagnosis of the toxic effect of silver nanoparticles encapsulated in the natural polymeric matrix of arabinogalactan (nAgAG). Laboratory animals of the experimental group for 9 days intragastrically injected aqueous solution of nAgAg at a rate of 100 mcg of silver per kilogram of weight in 0.5 ml of distilled water. The animals of the control group receive 0.5 ml of distilled water for 9 days intragastrically. Immediately after the end of the exposure, sections of the brain tissues of the animals of both groups are made. They are stained for antibodies to the proteins caspase-3 and bcl-2 and conduct a survey microscopy of the preparations obtained. At this time, the total number of cells in 0.2 mm², the number of pathologically altered neurons with caspase 3 expression, the number of pathologically altered neurons with bcl-2 expression, the number of normal neurons with caspase 3 expression, the number of normal neurons with bcl-2 expression as a percentage of the total cell count of 0.2 mm². The ratio is calculated of these indicators on the drugs of the experimental group to those of the control group. When the number of pathologically altered neurons with caspase 3 expression increased by an average of 1.6 times in the experimental group, the number of normal neurons with the expression of caspase 3 averaged 2.5 times, pathologically altered neurons with bcl-2 expression on average of 2.4 fold, the number of normal neurons expressing bcl-2 is 1.5 times the conclusion about the presence of the process of apoptosis in the brain tissues of animals.

EFFECT: method makes it possible to evaluate the functional state of neurons of the brain under the influence of silver nanobiocomposites, expands the arsenal of methods for evaluating the effect of toxic substances on the organism of laboratory animals.

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