METHOD OF ASSESSING THE TOXIC EFFECT OF A BIMETALLIC FERRUM-GADOLINIUM NANOCOMPOSITE ENCAPSULATED IN A NATURAL POLYMER MATRIX OF ARABINOGALACTAN ON LABORATORY ANIMALS Russian patent published in 2020 - IPC G09B23/28 B82B1/00 

FIELD: medicine.

SUBSTANCE: invention refers to experimental medicine, general toxicology and nanotoxicology, and concerns criteria for diagnosing the toxic effect of bimetallic nanocomposites encapsulated in a natural polymer matrix of arabinogalactan. Method for assessing the toxic effect of a bimetallic ferrum-gadolinium nanocomposite encapsulated in a natural polymer matrix of arabinogalactan on laboratory animals consists in the fact that the toxicant to the animals of the experimental group is orally administered in dose of 500 mcg/kg for 10 days, distilled water to the animals of the control group is administered orally in 0.5 ml for 10 days, cerebral cortical sections are made, one part of sections of cerebral cortex tissues of animals of the control and experimental groups is stained with haematoxylin and eosin, the other – with antibodies to heat shock protein 70, observational microscopy of the obtained preparations is carried out, the astroglial cell count and the number of neurons with heat shock protein 70 expression are calculated per unit area of the section, the obtained indices of both groups of animals are compared and if the number of astraglial cells is decreased by 1.16 times and the number of sensorimotor bark neurons is increased with expression of the heat shock protein 70 by 11.5 times in the test group as compared to the control group concluding on development of pathological process in tissues of sensorimotor cerebral cortex under action of bimetallic ferrum-gadolinium nanocomposite, encapsulated in natural polymer matrix of arabinogalactan.

EFFECT: disclosed is a method of assessing the toxic effect of a bimetallic ferrum-gadolinium nanocomposite encapsulated in a natural polymer matrix of arabinogalactan.

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