MUTANT POLYMERASES OF HEPATITIS B VIRUS Russian patent published in 2017 - IPC C12N9/22 C12N5/10 C12N15/11 C12N15/63 C07K14/02 A61K39/29 A61P31/12 

FIELD: biotechnology.

SUBSTANCE: invention relates to biochemistry, in particular, to mutant HBV polymerase polypeptide containing a mutant HBV polymerase domain with the internal deletion that suppresses the polymerase functional activity, and mutant RNase H domain with the internal deletion and mutations that suppresses the functional activity of RNase H. This invention also relates to a hybrid protein containing the said mutant HBV polymerase polypeptide and core HBV polypeptide or immunogenic HBsAg envl and env2 domains. The said mutant polypeptide and hybrid proteins are used for induction or stimulation of the immune response against HBV. The present invention also discovers a nucleic acid molecule, an expression vector and a composition and kit for the treatment or prevention of the infection caused by HBV or HBV-associated diseases and of pathological conditions. The present invention also discloses the host-cell, a method of obtaining the hybrid proteins and a method of obtaining viral particles which uses the said expression vector.

EFFECT: invention provides for improving the immunizing capacity of mutant HBV polypeptides preserving security when using them.

46 cl, 11 dwg, 2 ex