FIELD: biotechnology.
SUBSTANCE: methods include plasma fractionation according to Cohn, and/or Kistler-Nitschmann to obtain an immunoglobulin-containing solution. Then, the immunoglobulin solution is contacted with a cation exchanger equilibrated at a pH of 3.8 to 5.3, for factor XI, factor XIa, and kallikrein binding, and with an anion exchanger equilibrated at a pH of 7 to 8.2, for prekallikrein binding. Then, the unbound immunoglobulin-containing fractions are collected. The resulting immunoglobulin composition comprises 4-10% of the total protein and is suitable for treating patients with immunodeficiency, inflammatory disease, autoimmune disease, or acute infection.
EFFECT: invention maximizes thrombogenic agents factor XI, factor XIa, kallikrein and prekallikrein removal from the immunoglobulin solution using a cation exchanger in a very narrow range of pH values, while maintaining a constant level of immunoglobulin in the solution.
49 cl, 16 tbl, 14 ex
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