FIELD: biotechnology.
SUBSTANCE: recombinant targeted toxin, specific for HER2 receptor expressing cells is proposed. The recombinant target toxin comprises a HER2-specific targeting module represented by the HER2-specific darpin DARPin class coding sequence and a toxic module in the form of a fragment of pseudomonade exotoxin A ETA interconnected by a flexible hydrophilic 16-amino acid linker represented by the sequence of SEQ ID NO: 1, oligogistidine sequence His6 and KDEL sequence at the C-terminus of the molecule. The recombinant target toxin is represented by the sequence of SEQ ID NO: 2.
EFFECT: increased toxic effect on the HER2 receptor expressing cells and increased effectiveness of targeted therapy.
2 cl, 6 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT IMMUNOTOXIN, SPECIFIC TO THE CELLS EXPRESSING HER2 RECEPTOR | 2014 |
|
RU2576232C1 |
| METHOD OF PRODUCING RECOMBINANT ANTI-TUMOR TOXIN BASED ON PROTEIN BARNASE-BARSTAR AND TARGETED POLYPEPTIDE DARPIN WITH EFFECT OF INSTANT CANCELLATION OF CYTOTOXIC ACTION | 2015 |
|
RU2610179C1 |
| RECOMBINANT PROTEIN EXPRESSION IN DOUBLE-STRAND C FORM | 2006 |
|
RU2412253C2 |
| METHOD FOR CONJUGATION OF HUMAN HEAVY CHAIN CONSTANT FRAGMENT AND PEPTOID ANALOGUE OF AUTOANTIGEN MOG35-55 FOR MULTIPLE SCLEROSIS THERAPY | 2018 |
|
RU2724714C1 |
| CONJUGATE OF LUMINESCENT SILICON DIOXIDE NANOPARTICLES WITH RECOMBINANT SINGLE-DOMAIN C7B LAMA NANO-MONOANTIBODIES, CAPABLE OF SPECIFICALLY INTERACTING WITH RECOMBINANT HER2/NEU PROTEIN - ANALOGUE OF EPIDERMAL GROWTH FACTOR NATURAL RECEPTOR EGFR/ERBB OF HUMAN CELLS | 2017 |
|
RU2679075C1 |
| RADIONUCLIDE DIAGNOSTIC TECHNIQUE FOR BREAST CANCER WITH Her2/neu HYPEREXPRESSION | 2019 |
|
RU2720801C1 |
| RECOMBINANT IMMUNOTOXIN AIMED AT MESOTHELIN | 2012 |
|
RU2600067C2 |
| TECHNETIUM-99m COMPLEX WITH RECOMBINANT ADDRESSED PROTEIN MOLECULES WITH ANKYRIN REPEATS FOR RADIONUCLIDE DIAGNOSTICS OF MALIGNANT LANDSCAPE WITH HER2/NEU OVEREXPRESSION AND METHOD OF ITS OBTAINING | 2023 |
|
RU2812633C1 |
| MODIFIED PROTEINS, CONSTRUCTED TOXINS AND METHODS FOR PRODUCTION THEREOF | 2002 |
|
RU2305684C2 |
| METHOD FOR ELIMINATING UNDESIRABLE TARGET CELLS | 1997 |
|
RU2182493C2 |
