RECOMBINANT PROTEIN EXPRESSION IN DOUBLE-STRAND C FORM Russian patent published in 2011 - IPC C12P21/04 C07K14/195 A61K38/02 A61P31/00 

FIELD: chemistry.

SUBSTANCE: polypeptides or proteins in double-strand form are obtained via recombinant expression in E. coli host cells. The polypeptide or protein exhibits its biological activity in form of a double-strand polypeptide or protein; C-terminal amino acid group of the first strand is an Arg or Lys'groups; the second protein/polypeptide strand has in its N-end 1-20 amino acid groups and a VPXGS (PRS) pentapeptide sequence, where X denotes any naturally occurring amino acid, where V - Val, Leu, He, Ala, Phe, Pro or Gly, P - Pro, Leu, He, Ala, Phe, Val or Gly, G - Gly, Leu, lie, Ala, Pro, Phe or Val, and S denotes Ser, Tyr, Trp or Thr. The method of obtaining the disclosed product involves the following steps: modification of the polypeptide or protein on the nucleic acid level, so that said polypeptide or protein in its modified form contains a VPXGS sequence in its loop area, where X, V, P, G and S are as defined above; incorporation of a structure modified at the nucleic acid level into the E. coli cell; culturing and then lysis of host cells and separation of the double-strand polypeptide or protein which is, for example, a botulinum neurotoxin, particularly type A botulinum neurotoxin (BoNT(A)).

EFFECT: obtained proteins and polypeptides provide cytotoxic effect in small dosages.

23 cl, 9 dwg, 7 ex