FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry, biotechnology and medicine, namely to method of producing recombinant anti-tumor toxin-chimeric bifunctional protein, consisting of targeted polypeptide Darpin 9-29, specific to histochemical marker HER2/neu, and highly active bacterial ribonuclease barnase, connected with flexible peptide linker. For implementation of this method recombinant strain is produced Escherichia coli BL21(DE3)/pETDa by embedding into strain of E.coli BL21(DE3) plasmid pETDa. Said plasmid is produced by cloning of recombinant DNA fragment, coding said chimeric protein, in plasmid vector pET22 at restriction sites NdeI/HindIII.
EFFECT: present invention enables to obtain targeted protein characterized by low risk of side effects due to instant cancellation of cytotoxicity for targeting on HER2/neu therapy of tumors overexpressing HER2 receptor.
3 cl, 8 dwg, 3 tbl, 11 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT SARS-CoV-2 S PROTEIN RBD-BINDING PROTEIN | 2022 |
|
RU2778942C1 |
| RECOMBINANT PLASMID PROVIDING SYNTHESIS OF BARNASE IN CELLS OF ESCHERICHIA COLI, ESCHERICHIA COLI STRAIN - PRODUCER OF BARNASE AND METHOD FOR PRODUCING BARNASE | 2017 |
|
RU2650871C1 |
| POLYPEPTIDE WITH ANTI-SARS-COV-2 ACTIVITY AND PROLONGED CIRCULATION TIME IN BLOOD FLOW | 2023 |
|
RU2822355C1 |
| CONJUGATE OF LUMINESCENT SILICON DIOXIDE NANOPARTICLES WITH RECOMBINANT SINGLE-DOMAIN C7B LAMA NANO-MONOANTIBODIES, CAPABLE OF SPECIFICALLY INTERACTING WITH RECOMBINANT HER2/NEU PROTEIN - ANALOGUE OF EPIDERMAL GROWTH FACTOR NATURAL RECEPTOR EGFR/ERBB OF HUMAN CELLS | 2017 |
|
RU2679075C1 |
| METHOD OF IMMUNOFLUORESCENT DETERMINATION OF ANTIGENS LOCALISATION | 2006 |
|
RU2350959C2 |
| RECOMBINANT TARGET TOXIN SPECIFIC TO HER2 RECEPTOR EXPRESSING CELLS | 2016 |
|
RU2627215C1 |
| STRAIN OF SUSPENSION CULTURE OF VEGETABLE CELLS NICOTIANA TABACUM (SV, SAMSUN) AS PRODUCER OF RECOMBINANT ANTIBODIES TO HUMAN SPLEEN FERRITIN | 2002 |
|
RU2227808C1 |
| TECHNETIUM-99m COMPLEX WITH RECOMBINANT ADDRESSED PROTEIN MOLECULES WITH ANKYRIN REPEATS FOR RADIONUCLIDE DIAGNOSTICS OF MALIGNANT LANDSCAPE WITH HER2/NEU OVEREXPRESSION AND METHOD OF ITS OBTAINING | 2023 |
|
RU2812633C1 |
| RECOMBINANT PLASMID DNA pSC13D6 CONTAINING GENE OF ONE-CHAINED ANTIBODY TO TICK-BORNE ENCEPHALITIS VIRUS, AND STRAIN OF BACTERIA Escherichia coli BL21 (DE3)/pSC13D6-PRODUCER OF ONE-CHAINED ANTIBODIES TO TICK-BORNE ENCEPHALITIS VIRUS, EXPRESSING VIRUS NEUTRALISING PROPERTIES | 2008 |
|
RU2378378C1 |
| RECOMBINANT PLASMID DNA pEFN877, DETERMINING EXPRESSION OF THE HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE SURFACE OF ESCHERICHIA COLI CELLS, AND STRAIN OF BACTERIA ESCHERICHIA coli BL21(DE3)pLysS/pEFN877 - PRODUCER OF HYBRID POLYPEPTIDE WITH PROPERTIES OF 10-TH FIBRONECTIN DOMAIN ON THE CELLS SURFACE | 2015 |
|
RU2606014C1 |
