FIELD: medicine.
SUBSTANCE: for production of cytologic preparations of metaphase chromosomes, 1-2 ml of a suspension of fixed cells derived from the bone marrow or peripheral blood of the patient is applied to a horizontally held slide with a film of 55% aqueous acetic acid solution during hemopoietic cells karyotypes analysis. As a result, the content of metaphase plates with adequate chromosome spreads in cytological preparations is increased by at least 3 times.
EFFECT: invention provides fast and accurate analysis of metaphase chromosomes karyotype.
1 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR OBTAINING PREPARATIONS OF METAPHASE CHROMOSOMES OUT OF BONE MARROW CELLS IN HEMOBLASTOSESSUFFERING PATIENTS | 2000 |
|
RU2200318C2 |
METHOD FOR MAKING METAPHASE PREPARATIONS WITH WEAK CHROMOSOME CONDENSATION | 2008 |
|
RU2390776C1 |
METHOD FOR MAKING PREPARATIONS OF MIOTIC METAPHASAL CHROMOSOMES | 0 |
|
SU1732222A1 |
METHOD OF CULTIVATION OF AMNIOTIC LIQUID CELLS | 2016 |
|
RU2630658C1 |
METHOD FOR PREVENTION AND CORRECTION OF CYTOGENETIC DISORDER | 2012 |
|
RU2504392C1 |
METHOD OF OCCUPATIONAL SELECTION OF PERSONS FOR WORK ON DESTRUCTION OF CHEMICAL WARFARE AGENTS | 2016 |
|
RU2642603C2 |
RECOMBINANT PLASMID DNA pYAII-39, USED FOR 4TH AND 9TH HUMAN CHROMOSOME MARKING IN METAPHASIC AND INTERPHASIC CELLS | 2006 |
|
RU2325441C1 |
METHOD OF DETERMINING GENOTOXICITY OF NANOPARTICLES | 2019 |
|
RU2711990C1 |
RECOMBINANT PLASMID DNA ALPHA-R-12 DESIGNATED FOR MARKING 6-TH HUMAN CHROMOSOME IN METAPHASE IN INTERPHASE CELLS | 2004 |
|
RU2265060C1 |
METHOD OF MARKING HUMAN 7TH CHROMOSOME IN METAPHASIC AND INTERPHASIC CELLS | 2009 |
|
RU2425890C2 |
Authors
Dates
2017-08-09—Published
2016-09-15—Filed