FIELD: medicine.
SUBSTANCE: method for making metaphase preparations with weak chromosome condensation consists in free-running cell cultivation, either short-range mitogen-free, or long-range with mitogen (mitogens), "night" colcemidisation for 16-18 h to enable fixation, hypotonisation, fixation and application of a cell suspension on a slide. A cell cycle is blocked with excess thymidine (5×10-7 M) during 17-18 h after cell pre-cultivation in a culture medium during 5-6 h mitogen-free, or 54 h with mitogen (mitogens). The cell cycle is cleared of the blocker by double washing in the culture medium. Reincubation is carried out with 2 mcg/ml of ethidium bromide added during 23-24 h. In colcemidisation, colcemid is concentrated 0.02 mcg/ml. It is carried out 6-8 h after the beginning of incubation with ethidium bromide added.
EFFECT: more rapid and more accurate karyotype analysis.
2 ex
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