FIELD: biotechnology.
SUBSTANCE: polypeptide that induces formation of neutralizing antibodies to Clostridium difficile toxins, is characterized by an amino acid sequence represented by any of SEQ ID NO: 4 or SEQ ID NO: 6. This invention also relates to an immunogenic composition comprising an isolated polypeptide. The invention also relates to a recombinant Clostridium difficile cell. This recombinant cell is designed to express the isolated polypeptide. The invention relates to methods for Clostridium difficile culturing and for preparation of the said isolated polypeptide.
EFFECT: invention allows to obtain an isolated polypeptide and an immunogenic composition that cause formation of neutralizing antibodies to Clostridium difficile toxins.
39 cl, 59 dwg, 71 tbl, 46 ex
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