FIELD: chemistry.
SUBSTANCE: way of getting that includes cultivation of the polypeptide of Escherichia coli cells, containing nucleic acid encoding a polypeptide, regulation of pH values during cultivation using alkaline solution containing amino acid polypeptide selection of cells or cultural medium and, thus, obtaining a polypeptide. However, this concentration of amino acid in alkaline solution is higher than the solubility of amino acids in water at 20°C and at neutral pH. The concentration of amino acid in alkaline solution is 30 g/l or more. Alkaline solution represents a 10 % (weight/volume) or more of a solution of ammonia. Escherichia coli cell is a cell of auxotrophic on leucine and proline strains of Escherichia coli K12. Alkaline solution contains leucine and proline. Cultivating implement to achieve cell dry weight 20 g/l or more.
EFFECT: obtaining polypeptide with high yield.
8 cl, 4 dwg, 3 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING RECOMBINANT POLYPEPTIDE | 2011 |
|
RU2577972C2 |
| RATIONALLY DEVELOPED MEDIA FOR CELL CULTIVATION | 2007 |
|
RU2520810C2 |
| NEW SELECTION SYSTEM | 2006 |
|
RU2415935C2 |
| METHOD OF OBTAINING POLYPEPTIDE AND METHOD OF PRODUCING REDUCED AMOUNT OF GLYCOFORM G(0) AND/OR AMOUNT OF GLYCOFORM G(1) OF POLYPEPTIDE | 2011 |
|
RU2595407C2 |
| METHOD FOR PREPARING L-AMINO ACIDS USING MICROORGANISM BELONGING TO Escherichia GENUS WHEREIN yafA GENE IS INACTIVATED | 2005 |
|
RU2315808C2 |
| L-ASPARATATE OXIDASE VARIANT AND METHOD FOR PRODUCING QUINOLINATE OR NICOTINIC ACID USING SAME | 2014 |
|
RU2648167C2 |
| IMPROVED CELL CULTURE MEDIUM | 2011 |
|
RU2563353C2 |
| IMPROVED CELL CULTURE MEDIUM | 2011 |
|
RU2798069C2 |
| METHODS FOR PRODUCING DOUBLE-STRANDED PROTEINS IN BACTERIA | 2015 |
|
RU2739500C2 |
| METHODS FOR PRODUCING DOUBLE-STRANDED PROTEINS IN BACTERIA | 2015 |
|
RU2705274C2 |
