FIELD: biotechnology.
SUBSTANCE: presented strain was derived from the protective Bacillus anthracis antigen by inserting the pGEM-TTPPAG plasmids into the E. coli strain BL21 (DE3). The plasmid pGEM-TTPPAG contains a T7 phage promoter that controls the expression of a fusion gene that is a full-length TTR bacteriophage DT57C gene (encodes the pb6 tail tube protein) fused at the 3'-end with the B. anthracis protective antigen gene and expressed in the form of virus-like particles. The obtained HPV, free of soluble cell protein, can be used as an antigen for immunization.
EFFECT: increasing the yield of the target product under optimal cultivation conditions, obtaining the main protective antigen of the causative agent of the anthrax in the form of virus-like particles, which facilitates its purification and potentially increases immunogenicity.
5 dwg, 3 tbl
| Title | Year | Author | Number |
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| Ecoli BL21 (DE3)pLysS CELL STRAIN, pTT9/ASFVp30 CLONE CONTAINING RECOMBINANT PLASMID WITH INTEGRATION OF PART OF CP204L GENE OF AFRICAN SWINE FEVER VIRUS, ENCODING CONFORMATIONAL EPITOPE OF PROTEIN p30, FOR PRODUCING DIAGNOSTIC PREPARATIONS | 2011 |
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RU2642323C2 |
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RU2333956C1 |
| RECOMBINANT PLASMID DNA PET21-NPVE CONTAINING NUCLEOLOPROTEIN (NP) GENE OF EBOLA VIRUS AND RECOMBINANT PROTEIN NP-VE, OBTAINED AS RESULT OF EXPRESSION OF NP GENE OF EBOLA VIRUS USING RECOMBINANT PLASMID DNA PET21-NPVE AND POSSESSING IMMUNOGENIC AND ANTIGENIC PROPERTIES | 2020 |
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| DNA SEQUENCE OF APTAMERS BINDING SHIGA-TOXIN OF TYPE 2 | 2014 |
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