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IPC

C12N1/21(2006-01-01)

C12R1/19(2006-01-01)

(21) (22)

Application

2016122721, 2016-06-09

(24)

Start date

2016-06-09

(22)

Actual filing date

2016-06-09

(45)

Published

2018-01-24

(72)

Inventor

Deryabina Yuliya IvanovnaIsakova Elena PavlovnaKrasnikov Boris FedorovichAntipov Aleksej NikolaevichBelyakova Alla VladimirovnaBiryukova Yuliya Konstantinovna

(73)

Holder

Federalnoe Gosudarstvennoe Uchrezhdenie Issledovatelskij Tsentr Osnovy Biotekhnologii" Rossijskoj Akademii Nauk" Biotekhnologii Ran)

RECOMBINANT PRODUCER OF HUMAN OMEGA-AMIDASE Nit2 BASED ON ESCHERICHIA COLI Russian patent published in 2018 - IPC C12N1/21 C12R1/19

Abstract RU 2642323 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to a recombinant strain of Escherichia coli BL21 (DE3; pQE-Nit2). This strain was obtained by transforming the Escherichia coli strain BL21 (DE3) by the plasmid construct pQE-Nit2, which is under the control of the T5 phage promoter and is designed to produce human ω-amidase (Nit2) when cultivated on nutrient media based on peptone, yeast extract and glucose. The peculiarity of the Nit2 gene in the pQE-Nit2 construct is optimized codon composition, as well as presence of an artificially introduced 6His- affinity tag immediately after the initiator codon ATG (Met), which allows one-step product purification by metal-affinity chromatography on Ni-NTA-agarose.

EFFECT: invention allows to obtain human ω-amidase with an elevated yield.

2 dwg, 1 tbl, 6 ex

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RU 2 642 323 C2

Authors

Deryabina Yuliya Ivanovna

Isakova Elena Pavlovna

Krasnikov Boris Fedorovich

Antipov Aleksej Nikolaevich

Belyakova Alla Vladimirovna

Biryukova Yuliya Konstantinovna

Dates

2018-01-24—Published

2016-06-09—Filed