FIELD: biotechnology.
SUBSTANCE: following has been proposed: a recombinant plasmid genetic construct pET21-GST-CD providing the synthesis and secretion of the Gc protein, the ectodomain of the Hantaan virus glycoprotein, having the following nucleotide sequence SEQ ID NO: 1 of 7,449 bp. in size. The following is also proposed: a recombinant strain of Escherichia coli BL21(DE3)pLysE pET21-GST-CD, a producer of the specified Gc protein obtained by transformation with the specified plasmid and deposited in the Collection of Bacteria, Bacteriophages and Fungi of the State Scientific Center for VB "Vector" of Rospotrebnadzor under registration number B-1386.
EFFECT: inventions ensure the production of the full-length ectodomain of the Gc glycoprotein of Hantaan virus.
2 cl, 4 dwg, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| pET21-GST-CD RECOMBINANT PLASMID WHICH ENSURES SYNTHESIS AND SECRETION OF Gn ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN, AND BL21(DE3)PLYSE pET21-GST-CD RECOMBINANT ESCHERICHIA COLI STRAIN, PRODUCER OF Gn PROTEIN, ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN | 2023 |
|
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