pET21-GST-CD RECOMBINANT PLASMID WHICH ENSURES SYNTHESIS AND SECRETION OF Gn ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN, AND BL21(DE3)PLYSE pET21-GST-CD RECOMBINANT ESCHERICHIA COLI STRAIN, PRODUCER OF Gn PROTEIN, ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN Russian patent published in 2023 - IPC C12N15/33 C12N15/70 C12N1/21 A61P31/14 C12R1/19 

Abstract RU 2809199 C1

FIELD: biotechnology.

SUBSTANCE: following has been proposed: a recombinant plasmid genetic construct pET21-GST-CD providing the synthesis and secretion of the Gn protein, the ectodomain of the Hantaan virus glycoprotein, having the following nucleotide sequence SEQ ID NO: 1 of 7,398 bp. in size. The following is also proposed: a recombinant strain of Escherichia coli BL21(DE3)pLysE pET21-GST-CD, a producer of the specified Gn protein obtained by transformation with the specified plasmid and deposited in the Collection of Bacteria, Bacteriophages and Fungi of the State Scientific Center for VB "Vector" of Rospotrebnadzor under registration number B-1385.

EFFECT: inventions ensure the production of the full-length ectodomain of the Gn glycoprotein of Hantaan virus.

2 cl, 4 dwg, 4 ex

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RU 2 809 199 C1

Authors

Isaeva Anastasiya Aleksandrovna

Shcherbakov Dmitrij Nikolaevich

Yashina Lyudmila Nikolaevna

Dates

2023-12-07Published

2023-03-21Filed