FIELD: biotechnology.
SUBSTANCE: following has been proposed: a recombinant plasmid genetic construct pET21-GST-CD providing the synthesis and secretion of the Gn protein, the ectodomain of the Hantaan virus glycoprotein, having the following nucleotide sequence SEQ ID NO: 1 of 7,398 bp. in size. The following is also proposed: a recombinant strain of Escherichia coli BL21(DE3)pLysE pET21-GST-CD, a producer of the specified Gn protein obtained by transformation with the specified plasmid and deposited in the Collection of Bacteria, Bacteriophages and Fungi of the State Scientific Center for VB "Vector" of Rospotrebnadzor under registration number B-1385.
EFFECT: inventions ensure the production of the full-length ectodomain of the Gn glycoprotein of Hantaan virus.
2 cl, 4 dwg, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID pET21-GST-CD WHICH ENSURES THE SYNTHESIS AND SECRETION OF Gc ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN, AND THE RECOMBINANT ESCHERICHIA COLI STRAIN BL21(DE3)pLysE pET21-GST-CD, A PRODUCER OF THE Gc PROTEIN, THE ECTODOMAIN OF HANTAAN VIRUS GLYCOPROTEIN | 2023 |
|
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